Efficacy And Safety Assessment Of A Novel UVC Device For Treating Corneal Bacterial Infections

1. Simon J Dean^1,2
2. Alex Petty¹
3. Simon Swift¹
4. Jennifer J McGhee¹
5. Sunil Shah^2,3
6. Jennifer P Craig¹

¹University of Auckland, Auckland New Zealand
²University of Ulster, Northern Ireland, United Kingdom
³Birmingham and Midlands Eye Centre, Birmingham, United Kingdom

Purpose: A prototype solid-state Ultraviolet-C (UVC) LED device may be useful in the treatment of corneal microbial infections, as UVC is commonly used for eradicating bacteria, fungi and viruses in other settings. This study assessed the efficacy of 265nm UVC from this LED, on four different bacterial strains, and investigated the consequences of corresponding exposures on human corneal epithelial cells in vitro.

Methods: Agar plate lawns of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus pyogenes were exposed to a 4.5mm diameter 265nm UVC beam at a fixed intensity and distance, for 30, 5, 4, 2 and 1 seconds. Growth inhibition was assessed with a BioRad Gel imager, and the diameter of lucent areas of bacterial inhibition recorded. Human corneal epithelial cells cultured on glass cover-slips were exposed to corresponding doses of UVC from the same device. Live/dead staining was performed and the results quantified.

Results: There was 100% inhibition of growth for all bacteria tested, at all exposure times. A 30-second exposure of human corneal epithelium to UVC gave no statistically significant decrease (p=0.877) in the ratio of live to dead cells when compared to control cultures.

Conclusion: The results confirmed that a 1 second exposure to germicidal UVC from this LED source was sufficient to inhibit microbial proliferation in the four bacterial strains tested in vitro. The literature suggests UVC at this dose could potentially be beneficial in treating corneal surface infections, without causing significant adverse effects, supported by our findings in human corneal epithelium exposed to UVC.