Experimental Study on the Effect of Juhua Juiming Powder（Traditional Chinese Medicine） in Preventing High-risk Corneal Transplantation Immune Rejection in Rat

Dongli Ma

Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Purpose To study the inhibitory effect of Juhua Jueming powder (JJP, Traditional Chinese Medicine) on high-risk corneal transplantation immune rejection in rat.

Methods The high-risk corneal transplantation immune rejection rat model was established by inducing coneal neoangiogenesis by suture method and the penetrating transplantation. Model rats were divided into two groups, the treated group and the control group, they were administered with JJP 0.1 g (dissolved in 2 ml water) and saline respectively via gastric infusion every day after transplantation. The survival status of the allograft, histopathology, local and systemic immune status in the recipients were observed using immunofluorescence histochemistry and flow cytometry.

Results The survival time of the allograft in the treated group (14.50±3.55 days) was significantly longer than that in the control group (8.25±0.71 days, P<0.01). Levels of Fas, FasL expressions in iris were stronger, and the percentage of CD4CD25FOXP3 positive cells in peripheral blood was less (5.11±3.92% vs 14.81±2.58%) in the control group than those in the treated group respectively. The concentration of IL-2 was lower while that of IL-10 was higher in aqueous humor of the treated group than those in the control group, respectively.

Conclusions JJP has certain effect for preventing high-risk corneal transplantation immune rejection in rat model.