The Effect of Estrogen on Adenoid Cystic Carcinoma
Lacrimal Gland Cell Culture proliferation

1. Elfina G. Sadono
2. Nina Handayani

Saiful Anwar Hospital, Malang, Indonesia

Purpose: To evaluate the effect of estrogen (estradiol valerate) on adenoid cystic carcinoma (AdCC) lacrimal gland cell culture proliferation.

Methods: Adenoid cystic carcinoma tumor of lacrimal gland was cultured in DMEM and F12 ham medium. Estrogen was given with concentration 0, 20 pg/ml,20o pg/ml and 20 ng/dl. Cells proliferation were counted at 0, 24, 48 hour. Anova test were used for statistical analysis.

Result: Cell count at 24 hours found that the proliferation were highest in AdCC cell culture with estrogen 20 pg/ml (average 348.330 cells/ml), and decreased in culture with estrogen 200 pg/dl average 346.670 cells/ml), and estrogen 2A ng/ml (average 318.330 cells/ml). Cell count in control group at 24 hour were average 172.000 cells/ml. The difference were not statistically significant (p=0-460). While cell count at 48 hours found that the proliferation were highest in AdCC cell culture with estrogen 20 ng/dl (average 705.000 cells/ml), followed by estrogen 200 pg/dl (average 531.660 cells/ml), and estrogen 20 pg/ml (average 458.330 cells/ml). Cell count in control group at 48 hour were average 398.330 cells/ml. The difference were statistically significant in estrogen 20 ng/ml (p=0.043).

Conclusion: Estrogen (estradiol valerate) 20 ng/ml had a significant effect on stimulation of AdCC lacrimal gland tumor culture cells proliferation after 48 hours