Inhibitory effects of Sodium 1-(12-Hydroxy) octadecanyl Sulfate,an MMP-2 inhibitor,on the development of defocus-induced myopia in guinea pigs

Yang Jiang

Objective: To investigate the inhibitory effects of Sodium 1-(12-Hydroxy) octadecnyl Sulfate,an MMP-2 inhibitor ,on the development of defocus-induced myopia in guinea pigs and its mechanism.

Methods: Thirty newborn guinea pigs were randomly divided into 3 groups. group A :-10D lens applied on right eye; group B :-10D lens and MMP-2 inhibitor treatment in right eye (9.0μg/ml,subconjunctival injection,every 3 days interval); group C: -10 D lens and vehicle treatment in right eye (same volume and frequency with those of group B ); there was no treatment in all the left eyes in this study . After 28 dys of defocus, the refractive status was recorded and the posterior scleral sample was collected for MMP-2 zymography and western-blot analysis.

Result: The treated eyes of group A,B and C gained (5.73±0.36)D,(2.58±0.33)D and (6.31±0.73)D respectively, the amount of gained myopic refraction in eyes of group A was significantly less than that of eyes in group B and group C (p<0.05); zymography analysis indicated that,of all the defocused animals ,the activity of MMP-2 in the eyes treated by inhibitor was significantly lower than that of eyes treated by vehicle and no injected eyes,represented by the lower level of active MMP (p<0.0.5);no significant difference was detected in the latent MMP-2 level between three groups (p>0.05).the MMP-2 protein level in posterior sclera of three groups also didn't show any signnificant difference by western-blot analysis(p>0.0.5).

Conclusions: 1-(12-Hydroxy) octadecanyl Sulfate(9.0μg/ml,subconjunctival injuection,every 3 days interval) inhibited the development of defocus –induced myopia in newborn guinea pigs,may through,at least in part,inhibitory effects on the activity of MMP-2 in posterior sclera.