Fig. 27. Using the technique of quick-freeze/deep-etch, the complexity of the matrix in the juxtacanalicular region is evident (JCT). A giant vacuole is seen (GV) with a pore (arrowhead) opening into the lumen of Schlemm's canal (SC). Specimen from an 81-year-old normal donor eye perfusion fixed at 45 mm Hg intraocular pressure (IOP).