Fig. 16 Complex granules resulting from interactions between melanosomes and various parts of the lysosomal system. A. Melanolipofuscin granules (Mlf) have a core of melanin and a cortex of lipofuscin material. The bleb (Ly) on the side of a lipofuscin granule (Lf) has been shown in other studies to contain lysosomal enzymes (×43,000). B. Transmission electron micrograph showing the sequestration of many melanosomes, presumably representative of autophagic digestion. Left. Routine preparation on an 84-year-old eye (×10,500). Right. Acid phosphatase preparation on a 22-year-old eye shows the enzyme activity within the matrix of this macrolysosome. Melanosomes are in various stages of disassembly (BrM, Bruch's membrane; ×11,500).