|
|
|
|
|
|
| Therapeutic effect on intraocular melanoma in mice with IL-12 gene modified tumor cell vaccine combinated with CpG-ODN |
|
| 作者：Zhao jun… 文章来源： Shenzhen eye hospital（深圳市眼科医院）,518001 点击数1870 更新时间：2006/6/30 15:15:47 文章录入：christinezeng 责任编辑：毛进 |
|
|
|
| Objective To observe the therapeutic effect on IL-12 gene modified tumor cell vaccine combinated with CpG oligodeoxynucletide（CpG-ODN）. Methods Model mice bearing tumor were divided into 3 groups, respectively phosphate-buffered saline (PBS) treatment group, IL-12 gene modified tumor cell vaccine (GTV) treatment group and GTV combinated with CpG-ODN (GTVC) treatment group. 30μl PBS, 30μl（3.0×106cells）inactivated GTV and GTVC containing 20μg CpG-ODN were respectively injected into subconjunctiva of mice bearing tumor 3 days and 10 days after bearing tumor mouse model had been established. The time of cornea diabrosis, metastases into cervical lymph nodes and lung, survival time and ratio of CD4+and CD8+T cells in peripheral blood were observed. Results The time of cornea diabrosis in GTVC and GTV groups was later than that in PBS group, but no statistical difference between GTVC and GTV group. Metastases in cervical lymph nodes of all mice were developed in PBS group, 25% mice in GTV group and in GTVC group 18 days after the model had been established. There was statistical difference in metastatic incidence respectively between PBS group and other groups. There was no statistical difference in metastatic incidence between GTV group and GTVC group. Metastases in lungs of all mice were developed in PBS group, 75% mice in GTV group and 13% mice in GTVC group 28 days after the model had been established. There was statistical difference in metastatic incidence respectively between GTVC group and other groups. Compared with negative control (NC) group, the ratio of CD4+and CD8+T cells in peripheral blood was lower in PBS and GTV groups. There was no statistical difference on the ratio between GTVC group and NC group. The survival time in GTVC group was longer than that in PBS and GTV groups, but no difference between PBS group and GTV group. Conclusions CpG-ODN can enhance the anti-tumor effect of IL-12 gene modified tumor cell vaccine. |
|
|
|
|
|
|
|
|
|
