|
|
|
|
|
|
| Fumintide Suppresses Retinal Neovascularization in Hypoxia Induced Mice |
|
| 作者：xiaqingj… 文章来源：West China Hospital，Sichuan University， Chengdu, 610041 P.R.China. 点击数1030 更新时间：2006/6/30 19:15:48 文章录入：xiaqingjie 责任编辑：毛进 |
|
|
|
| BCKGROUND Vascular endothelial growth factor (VEGF) plays a central role in the development of retinal neovascularization and diabetic macular edema. Fumintide (Kanghong Co. Chengdu, P.R.China) is a humanized antibody-liked molecule designed to bind VEGF.This protein was suggested to supress the neovascularizations.
PURPOSE. To study the efficacy of fumintide on experimental retinal neovascularization in the new born mice eye.
METHODS. Fifty one-week-old Kunming stain mice were exposed to 75% oxygen for 5 days and then to room air for the retinal neovascularization model.Fifteen mice were treated with fumintide (i.p,25mg/kg )once every other day as theropy group, another 15 mice were given the same volume PBS as model controls, 10 mice of the same age kept in room air as negtive controls. In the day 21 after birth, one eye of every mice was used to spread retinal flatmount and the other was fixed for tissue section.FITC-dextran perfused retinal flatmount was performed and examined to assess the oxygen-induced changes of retinal vessels.The proliferated neovascular response was quantitated by counting the nuclei of endothelial cells of new vessels extending from the retina into the vitreous in 6 μm sagittal cross sections.The data were analyzed with SPSS10.0.
RESULTS. Much constriction and closure of the blood vessels and fluoroscein leakage were found in the hyperoxia model controls, but not found in the control group and less in the fumintide treated group ; proliferation were found under the relatively hypoxia status.There was a mean of 37 neovascular nuclei per cross-section in the oxygen-treated retina and 5 nucleus in the fumintide treated group (P＜0.05) and less than 1 in the control group (P＜0.001)
.
CONCLUSIONS. This study demonstrates that fumintide has a strong supression on retinal neovascularization and profiles its clinical use in treating retinal neovacularization diseases.
【Key words】 Retinal diseases; Retinal Neovascularization;
Fumintide,
|
|
|
|
|
|
|
|
|
|
