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| The expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells |
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| 作者：CHEN Bo,… 文章来源：From the Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Technology and Science, Wuhan 430022, Hubei Province, China 点击数3390 更新时间：2006/7/10 15:33:41 文章录入：hamburgerl 责任编辑：毛进 |
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| [Abstract] Objective: To study the expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells(BTMC). Methods: Reanimating the pEGFP-C1 strain, pEGFP-C1 was extracted and identified. The pEGFP-C1 was embedded in lipofectamine and transfected into BTMC. The fluorescence intensity of BTMC after transfected with EGFP gene was visualized by fluorescence microscope. Results: pEGFP-C1 could be availably transfected into BTMC. After being transfected, BTMC with the EGFP gene displayed an effective EGFP expression. Conclusions: An EGFP expressing plasmid was used to transfected BTMC. Transfected cells show a stable EGFP expression in vitro. This labeling method will be profited for studying BTMC through genic level. |
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