| 目的：研究槲皮素（quercetin,QU）对兔Tenon囊成纤维细胞增殖的影响，探讨其作用的可能机制。方法：用组织块培养法培养兔Tenon囊成纤维细胞，取第3～5代细胞随机分A－F组6组，每组12孔。A－D组分别加入含5、10、20、50μmg／μl QU的培养液200μl，E组为丝裂霉素C（mitomycin C,MMC）对照组，加入终浓度为100mg／L的MMC，F组为空白对照组。各组继续培养24、36、48、72小时后采用MTT法测定不同浓度的QU对兔Tenon囊成纤维细胞的抑制作用，划线法观察细胞迁移，用流式细胞仪检测QU对兔Tenon囊成纤维细胞细胞周期的影响。结果：QU能浓度时间依赖性抑制兔Tenon囊成纤维细胞增殖。流式细胞仪显示，处理48小时后，G1期细胞增加了26.92％，S期细胞下降了23.50％，G2期细胞下降了3.42％。结论：QU可抑制兔Tenon囊成纤维细胞生长活性，有时间剂量效应。QU影响细胞周期各时相群体，抑制G1期向S期过渡，进而阻止细胞向G2期过渡，从而抑制细胞增殖。
[Abstract]Objective:To study the inhibitive effects of quercetin (QU) on the fibroblasts proliferation of rabbit Tenon’s capsule and its mechanism. Methods: Cultured fibroblasts were exposed to different concentrations of QU solution and investigated by MTT assay . Using flow cytometer observe the effect of QU on cells cycle. Results:QU can suppress the proliferation of rabbit Tenon’s capsule fibroblasts in vitro and show a dose-time dependent tendency .Flow cytometer results show cells increase 26.92% in G1 phase,decreased 23.50% in S phase and 3.42% in G2 phase. Conclusions: QU can suppress the proliferation of rabbit Tenon’s capsule fibroblasts in vitro and show a dose-time dependent tendency. QU may effect all phase of cell cycle and inhibit cell proliferation by inhibit G1 phase transitting to S phase and G2 phase . |
