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| Induction of Heat Shock Protein 72 in RGCs in Rat Model of Acute Experimental Glaucoma After Heat Stress or Zinc Administration |
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| 作者：QING guo… 文章来源：Department of Ophthalmology, the Second Xiangya Hospital, Central South University, Changsha, Hunan 410011 点击数1678 更新时间：2004/5/30 文章录入：毛进 责任编辑：毛进 |
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| ABSTRACT Purpose: To investigate the dynamics of heat shock protein72 (HSP72) expression in retinal ganglion cells (RGCs) in rat model of acute experimental glaucoma treated with heat stress or intraperitoneal injection of zinc sulfate. Methods: Twenty-seven male Wistar rats were used to make acute glaucoma models. Five others served as normal control. Acute glaucoma models were made by intracameral irrigation in the right eyes of Wistar rats with balanced salt solution (BSS) at 102 mmHg (13.57kPa) for 2 hours. Nine model rats were killed at different intervals after intracameral irrigation without treatment，which served as damage control. Ten were treated with heat stress(40~42℃), and eight were used for zinc sulfate administration, two days posterior to intracameral irrigation. Treated model rats were sacrificed at designed intervals after treatment. Right eyes were enucleated immediately, and the retinas were dissected for Western blot. Results: No HSP72 was found in RGCs of normal Wistar rats. In damage control group, slight HSP72 was detected during 6~36 hours following intracameral irrigation. In heat shock group and zinc sulfate group, HSP72 was detected significantly expressed in RGCs. But the dynamics of HSP72 production were quite different in these two treated groups. In heat shock group, HSP72 appeared at the sixth hour after treatment, and increased gradually until its peak production emerged at the 48th hour. HSP72 vanished at day 8 after treatment. In zinc sulfate group, HSP72 emerged at the 24th hour after zinc administration, and peaked at the 72th hour posterior to treatment. HSP72 expression then decreased slowly, and disappeared at day 21 after treatment. Conclusion: HSP72 can be induced in RGCs of acute glaucoma rats with heat stress or zinc sulfate administration. But the dynamics of the HSP72 expression between these two groups was quite different. |
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