Aim  The ability to induce somatic cells to pluripotency by ectopic expression of defined transcription factors has transformed the future of regenerative medicine. Patient-specific iPS may be good resources for some ophthalmology disease.
Methods   we seperated human lens epithelial cells (hLECs) from patient, after cell reprogramming factors(sox-2,oct-4,klf-4) transformation, yielding induced pluripotent stem (iPS) cells with high reprogramming efficiencies.
Results  HLEC-derived iPS (HLE-iPS) cell colonies were indistinguishable from human embryonic stem (ES) cells with regards to morphology, gene pattern ,pluripotent marker expression, and their ability to generate all embryonic germ layers in vitro and in vivo. Further more, HLE-iPS can be differentiated into large quantities of lens progenitor-like cells with defined factors (Noggin BMP,FGF2), These cells expressed and accumulated lens-specific markers.
Conclusion  Our studies identify a high efficient procedure to generate lens cells from patient specific iPS.