Object Incomplete optic nerve (ON) injury models are widely used in ophthalmological research, specially the optic nerve crush injury (ONC) model. However, the main drawback of most methods described for optic nerve crush is that the injury cannot be quantified at the site of the trauma. In the present study, we described a new method to crush the optic nerve by using a specially designed and commercially available device, aiming for optimizing and improving the traditional methods.
Methods Through retinal morphology and electrophysiological functional tests, the effectiveness and stability of this model were evaluated.
Results HE stained retinal sections showed that compared with sham group ganglion cells in ONC group occurred gradually degeneration and apoptosis, cell number reduced, and all retinal neuron layers got thinning over time. Flash visual evoked potential (FVEP) showed that compared with sham group the latency and amplitude of P1 wave gradually decreased in ONC group.
Conclusion Yasargil aneurysm clip is expected to be widely used in the future study.  
目的 以视神经钳夹伤（ONC）模型为代表的视神经不完全损伤模型是眼科研究中常用的动物模型，但损伤程度的定量和造模标准化是关键。本研究拟采用一种已商品化、有明确力学标记的Yasargil脑动脉瘤夹制作定量的视神经不完全损伤模型，以期优化和改善传统的模型制作方法。
方法 在改良传统造模方法的基础上，应用Yasargil脑动脉瘤夹定量地钳夹大鼠的视神经，通过形态学、功能学等指标对该模型制作方法的损伤程度和稳定性进行评价。
结果 视网膜组织HE染色切片显示，ONC组较假手术组的节细胞随时间逐渐出现变性和凋亡的表现，节细胞数量减少，三级神经元层厚度均变薄。闪光视觉诱发电位（FVEP）显示，ONC组较假手术（Sham）组的P₁波随时间逐渐出现潜伏期延长和振幅降低。
结论 应用Yasargil脑动脉瘤夹可获得稳定、定量的视神经不完全损伤模型，有望在未来的视神经损伤研究中得到广泛的应用。