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| Role of CD11a and CD11b in corneal wound healing and inflammatory process |
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| 作者：C Wayne … 文章来源：Leukocyte Biology Section, Department of Pediatrics and Immunology, Baylor College of Medicine, Houston, TX 77030 点击数1481 更新时间：2004/6/15 文章录入：毛进 责任编辑：毛进 |
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| Objective: Inflammation plays an important role in corneal wound repair. We
examined contributions of CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), important
leukocyte integrins, in corneal epithelial wound healing in mice. Methods: The
central corneal epithelium (2 mm diameter) was demarcated and removed in
C57Bl/6 wild type, CD11a -/- and CD11b-/- mice. Epithelial healing was
digitally analyzed every 6 hours. The infiltration of neutrophils into wounds
was quantified under deconvolution microscopy. Enzyme-linked immunosorbent
assay (ELISA) was used to measure IL-1, IL-6, IL-8, MCP-1, MIP-2, and LIX in
corneal extracts. Results: In both CD11a-/- and CD11b-/- mice, neutrophil
emigration, cytokine levels and chemokine levels were significantly delayed,
peaking at 24 hours after wounding (p<0.01) compared to wildtype mice peaking
at 12 hours. In wild type, re-epithelialization occurred by 18-24 hours, but
was delayed (p<0.01) in both knockout mice by an additional 6-12 hours
(CD11a-/-) and 24-48 hours (CD11b-/-). Neutrophil numbers were markedly
reduced in CD11a-/- (p<0.01) but markedly elevated in CD11b-/- mice (p<0.01)
compared to wild type. Conclusions: LFA-1 is required for efficient
neutrophil emigration into cornea, but Mac-1 is not. Mac-1 appears to be
important in limiting the inflammatory response. Absence of either integrin
significantly delays re-epithelization.
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