| 【摘要】目的 在体外成功分离培养虹膜色素上皮细胞，并观察其在特定条件下转分化为晶体上皮细胞的现象。方法 采用酶辅助机械分离法分离并培养兔眼虹膜色素上皮细胞。传代过程中在DMEM培养基内加入苯硫脲、透明质酸酶、重组人成纤维细胞生长因子-4及 L-抗坏血酸-2-磷酸完成转分化过程。分别对培养起始和终末的细胞进行光镜、电镜观察和免疫组化染色鉴定。结果 成功分离并培养出原代的虹膜色素上皮细胞，经过特定条件的培养最终转分化为晶体上皮细胞，免疫组化鉴定虹膜色素上皮细胞和晶体上皮细胞均有其各自特异染色。结论 酶辅助机械分离法是进行虹膜色素上皮细胞原代培养较好的方法。在体外培养条件下，虹膜色素上皮细胞可转分化为晶体上皮细胞，而透明质酸酶、苯硫脲、L-抗坏血酸-2-磷酸、重组人成纤维细胞生长因子-4等因素可能参与了对上述转分化过程的调节。
The experimental study on the course of the transdifferentiation from iris pigmented epithelial cell to lens epithelial cell
【Abstract】Objective To accomplish the detachment and culture of IPE in vitro and to investigate the course of the transdifferentiation from iris pigmented epithelial cell to lens epithelial cell during the specific condition. Methods The IPE of rabbits were detached and cultivated according to the method of enzyme-assisted microdissection. Then the IPE were cultivated in the culture medium appending with PTU、Huase、rhFGF-4、Asc-2P and so on. Finally the initiative and the ultimate cells were respectively identified by microscope、electroscope and the method of immunohistochemistry. Results The IPE of rabbits were successfully detached and cultivated and the course of the transdifferentiation was observed. The IPE and LEC were identified by their particular reaction to immunohistochemistry. Conclusion It is a good method to detach IPE by enzyme-assisted microdissection. During in vitro culture, IPE can undergo the course of transdifferentiation and turn into LEC finally. This experiment suggest that those factors such as PTU、Huase、rhFGF-4、Asc-2P and so on may be involved the regulation of the course of transdifferentiation.
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