| ［摘要］
目的: 本研究依据培养的甲状腺相关性眼病患者眼眶成纤维细胞是否表达CD90分为两个亚群，分离这两个细胞亚群，分别进行肌成纤维诱导，研究它们在眼外肌纤维化中所起的不同的作用。
方法: 免疫磁分离法分离CD90+和CD90－的眼眶成纤维细胞，TGF-β1诱导CD90+和CD90－成纤维细胞亚群转化为肌成纤维细胞（MFB），免疫细胞化学法检测代表MFB标志的平滑肌动蛋白α-SMA的表达情况。
结果: 在TGF-β1的作用下， 48小时后部分CD90+细胞开始出现α-SMA弱表达，4天后大部分细胞呈阳性。CD90－细胞不表达α-SMA。
结论: CD90+与CD90－眼眶成纤维细胞具有不同的功能，CD90+细胞群具有转化为MFB的潜能，与眼外肌纤维化和细胞外基质积聚有关；CD90－成纤维细胞不能转化为MFB。
【Abstract】
Objective: Devided orbital fibroblasts from patients with thyroid-associated ophthalmopathy into CD90+ and CD90－ subsets respect to surface CD90 expression ,then determined whether CD90+ and/or CD90－fibroblasts were capable of differentiating into myofibroblasts.
Methods: Fibroblasts subset separation into CD90+ and CD90－ subsets was accomplished by three to four rounds of magnetic bead selection,then treated with recombinant human TGFβ1— an known inducer of the myofibroblastic phenotype ,α-SMA expression was assayed at 24 hours, 2 days, 4 days, and 6 days after treatment using immunohistochemistry. α-SMA was a characteristic marker of myofibroblasts.
Results: TGFβ1 treatments inducedα-SMA expression only in CD90+ orbital fibroblasts, CD90－ orbital fibroblasts were not capable ofα-SMA induction after TGFβ1 .
Conclusions: Obital fibroblasts are heterogeneous with respect to surface CD90 expression,and that separation into CD90+ and CD90－ subsets result in functionally distinct subpopulations, Only CD90+ orbital fibroblasts are capable of myofibroblast differentiation after treatment with TGFβ1，are related to the extraocular muscles fibrosis and GAG accumulation in the orbit.
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