| Purpose:To investigate whether bone marrow-derived cells are present in Mooren’s ulcer and involve in its destructive and regenerative disease course.
Methods:Tissue specimens were collected from three eyes of three patients with Mooren’s ulcer that underwent lameller keratectomy. Three normal donor limbal corneoscleras served as controls. Immunohistochemical staining patterns were analyzed by using antibodies: CD34 (marker for the hematopoietic progenitor cells and endothelium), c-Kit (marker for hematopoietic and stromal progenitor cells), STRO-1 (a differentiation antigen present on bone marrow fibroblast cells and on various nonhematopoietic progenitor cells).
Results:Strong positive CD34, c-Kit and STRO-1 cells were revealed in Mooren’s ulcer specimens, especially in the superficial stroma. A few weakly expressed CD34 stromal cells were seen in normal limbal cornea, but no immunoreactivity for c-Kit and STRO-1 was found.
Conclusion: Bone marrow-derived cells are present in Mooren’s ulcer and contribute to its destructive and regeneration process by synergizing with other factors. Specific therapeutic strategies that target the role of these cells in Mooren’s ulcer are anticipated. |
