| Abstract Objective To investigate gene and protein expression of thyrotropin-receptor in retro-orbital tissue with Thyroid associated ophthalmopathy (TAO) . Methods Removed retro-orbital tissue from TAO patients, RNA isolated by single-step method of Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction and determinated by reverse transcription polymerase chain reaction (RT-PCR) for TSH-RmRNA.Immunocytochemistry staining were performed, using a mouse polyclonal antibody directed against TSHR peptide. Results The rate of TSH-RmRNA expression in TAO retro-orbital tissue was 91%, in healthy people was 20%, the former was significantly higher than the later. The protein expression rate is 66.7%, and negative in controls. Conclusion Highly expression of TSHR mRNA and TSHR like protein in TAO retro-orbital tissue indicates that as a common antigen between orbit and thyroid,TSH-R plays a key role in the process of the oncoming of TAO.
摘要 目的 研究促甲状腺激素受体（TSHR）在甲状腺相关眼病（TAO）患者球后脂肪和结缔组织中的基因表达和蛋白表达，确定TSHR 在 TAO发病机制中的作用。 方法 经眼眶减压术获得TAO患者球后脂肪与结缔组织，提取总RNA，经RT-PCR 产物电泳观察TSHRmRNA 的表达。基因表达阳性者进行免疫组织化学染色，观察其蛋白表达。 结果 TAO 患者球后组织中TSHRmRNA 阳性表达率为91%，对照组的阳性表达率为20%，二者有统计学差异。TSHR蛋白的阳性表达率为66.7%,对照组无TSHR蛋白的表达。结论 TSHRmRNA在 TAO患者球后脂肪和结缔组织中高度表达，且有TSHR蛋白表达，而正常个体没有表达，可以证实TAO患者球后组织中存在有活性的TSHR蛋白，提示TSHR可能作为交叉抗原在TAO发病机制中起重要作用。
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