|
|
 |
专题栏目 |
 |
|
相关文章 |
|
|
|
|
|
 |
Multiocus Sequencing Typing of Pseudomonas aeruginosa from bacterial keratitis |
热 |
|
| Multiocus Sequencing Typing of Pseudomonas aeruginosa from bacterial keratitis |
|
作者：WangDan,… 文章来源：Beijing Institute of Ophthalmology, Beijing Tongren Ophthalmic Center,17 Hou Gou Lane,,Chong Nei Street Beijing 100005, China 点击数：1358 更新时间：2006/6/27 19:20:12 
|
|
| Abstract
Objective : To study on the genetic background of five isolates of Pseudomonas aeruginosa from bacterial keratitis.
Methods: P. aeruginosa strains was from five patients(five eyes),all female. The range of age was from ten to seventy-one. The strains were cultured on BHI agar at 37°C under aerobic conditions. Chromosomal DNA was extracted from these purified strains with a kit. PCR primers were designed specially for seven house-keeping loci: acsA, aroE, guaA,mulT,nuoD, ppsA, trpE. Amplification and sequencing of the loci, offer the sequencs to the website(http://www.pubmlst.org) The site assigned alleles at multiple house-keeping loci by sequencing. An arbitrary number was given to each distinct allele within a locus. Each isolate was therefore given seven numbers that represented its sequence type (ST).
Result:
PA564 (43-30-31-26-4-24-32) belonged to ST127; PA583(17-24-37-5-4-7-7)belonged to ST241; PA521(36-5-29-7-4-10-7) belonged to ST170; PA536 (38-11-34-13-1-2-4) belonged to ST235; PA351 (5-1-9-40-1-16-24) belonged to ST238.
Conclusions::
MLST is a highly discriminatory method which can be used to standardize for. Pseudomonas aeruginosa typing. The result is unambiguous .The typing can help us to learn etiology, pathogenesis of the pathogen more further It also can give us a clue to understand the phylogenetic relationships and background between isolates better.
|
|
|
| 会议投稿录入：vickysarah 责任编辑：毛进 |
|
|
上一篇会议投稿： 眼轴延长术—萎缩眼球的活动性义眼台植入
下一篇会议投稿： 开放性眼外伤中导致视力受损的并发症的临床分析 |
|
|
| 【字体：小 大】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 |
|
 网友评论：（只显示最新10条。评论内容只代表网友观点，与本站立场无关！） |
| |
|
|
|
|
