|
| Expression of the Photopigment Melanopsin in the Retinal Ganglion Cells in RCS rats during Retinal Degeneration |
|
作者：Zhongsha… 文章来源：Southwest Eye Hospital 400038 点击数：1356 更新时间：2006/6/30 11:14:21 
|
|
| PURPOSE
To investigate the morphology and distribution of melanopsin-containing retinal ganglion cells in retinal dystrophic RCS rats during retinal degeneration.
METHODS
1. Retinal dystrophic RCS rats and non-dystrophic RCS rats aged postnatal 21 days (P21d) and P60d were used for this study. Whole-mounting retina, frozen and paraffin sections were prepared for immunohistochemistry staining with anti-melanopsin antibody.
2. The retina of P21d and P60d retinal dystrophic and control RCS rats were dissected and western-blot was adopted to detect protein expression of melanopsin during different postnatal stages.
RESULTS
1. Immunohistochemistry staining showed anti-melanopsin antibody positive in about 2% RGCs in non-retinal dystrophic RCS rats and theses cells distributed in whole retina. There were not differences between retinal dystrophic and control RCS rats at P21d. But at P60d the number of melanopsin-positive RGCs reduced significantly in retinal dystrophic RCS rats.
2. Western-blot showed melanopsin expression was very weak in rats retina. The strap of 60kd protein appeared clearly in both control RCS rats and P21d retinal dystrophic RCS rats. But this strap was nearly invisible in P60d retinal dystrophic RCS rats, indicating melanopsin protein expression descreased dramaticly.
CONCLUSION
The expression of melanopsin protein decreased significantly at the late stage of retinal degeneration, and the number of melanopsin-containing RGCs disappeared following photoreceptors loss, suggesting that the functional protein expression in neural retina was impaired severely following photoreceptors loss at late stage of retinal degeneration.
|
|
|
| 会议投稿录入：chenlf5468 责任编辑：毛进 |
|
|
上一篇会议投稿： 怎样诊断眼眶炎症性病变？
下一篇会议投稿： 人胚胎视网膜移植到光诱导视网膜变性猪 |
|
|
| 【字体：小 大】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 |
|
网友评论：（只显示最新10条。评论内容只代表网友观点，与本站立场无关！）