|
|
 |
专题栏目 |
 |
|
相关文章 |
|
|
|
|
|
 |
The Role of Bone Marrow-derived Mesenchymal Stem Cells in Corneal Tissue Repair |
★★★ |
|
| The Role of Bone Marrow-derived Mesenchymal Stem Cells in Corneal Tissue Repair |
|
作者：Meidong … 文章来源：Save Sight Institute, Discipline of Clinical Ophthalmology and Eye Health, University of Sydney, Australia 点击数：238 更新时间：2011/9/13 
|
|
Objectives: Considering that there is a shortage of eye donor, the aim of corneal tissue engineering is to develop substitutes for the replacement of damaged tissues. Limbal stem cells have been considered as a source for continual renewal of corneal epithelium; however, it has limitation on the ocular surface disorders with limbal stem cell dysfunction. In addition, there is no reliable source for regeneration of corneal endothelium. This study is to investigate the ability of differentiation of bone marrow-derived mesenchymal stem cells (BMMSC) into corneal cells in vitro and their possible roles in corneal repair.
Methods: Bone marrow was collected from the femoral and tibial bones of WISTAR Albino rats.The MSCs were isolated and cultured with Ficoll Hypaque gradient method. The purification of MSCs was achieved by removal of nonadherent cells during subsequent changes of medium. Bone marrow derived cells were characterised for the presence of mesenchymal marker (CD29) and absence of hematopoietic marker (CD34) before performing experiments. MSC to epithelium and endothelium differentiation experiments were performed. The epithelial cells differentiated from BMMSCs were characterised by morphology and immunophenotyping using K3, ZO-1. The flow cytometry and Western blot were also used to confirm the cell differentiation. Epithelial and endothelial cell proliferation assays using various MSC mixed-cultures and MSC conditional medium were also conducted.
Results: Primary cultures of BMMSCs were obtained successfully. Immunohistochemistry study and flow cytometry analysis indicated that BMMSCs constantly expressed CD29 (mesenchymal marker). They were negative for CD34 (hematopoietic marker). In the induction of multi-lineage differentiation experiments, corneal epithelial cells were successfully differentiated from BMMSCs. Those cells were Cytokeratin 3 (corneal epithelial marker) and ZO-1 positive and CD 31 (endothelial marker) negative. The original MSC marker of CD29 was switched to negative on those differentiated epithelial cells. The endothelial-like cells were observed from the MSC cultures induced with corneal endothelial growth factor. The cells were ZO-1 positive. However they could not be characterised due to the lack of available specific corneal endothelial markers. SMCs showed promotion effects on corneal cell proliferation.
Conclusion: The present study demonstrates that bone marrow-derived mesenchymal stem cells have the ability to differentiate into corneal epithelial and endothelial-like cells. The study opens up the possibility of using these cells in corneal tissue engineering and regeneration in the treatment of corneal surface disorders.
This project is supported by Corneal Stem Cell Research Grant of Save Sight Institute and Dr McClellan’s Corneal Research Donation Fund.
|
|
|
| 会议投稿录入：毛进 责任编辑：毛进 |
|
|
上一篇会议投稿： 湖北省角膜疾病患者基本状况调查
下一篇会议投稿： 1%环孢霉素滴眼液治疗溃疡性坏死性角膜基质炎的疗效观察 |
|
|
| 【字体：小 大】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 |
|
 网友评论：（只显示最新10条。评论内容只代表网友观点，与本站立场无关！） |
| |
|
|
|
|
