| 目的 探讨外源性RA对小鸡后极部巩膜基质金属蛋白酶2（MMP-2）及其特异性组织抑制剂（TIMP-2）mRNA表达水平的影响。材料与方法 取1d龄来亨雏鸡，右眼球后注射RA一次，制备外源性RA实验组标本，对侧眼为自身对照组，阴性对照组右眼球后注射生理盐水，正常对照组小鸡不做任何处理。分别于注射后12h、24h、48h、72h摘取眼球，采用一步法逆转录－多聚酶链反应（RT-PCR法）检测每组小鸡后极部巩膜MMP-2与TIMP-2 mRNA表达水平。结果 与正常组、阴性对照组相比，实验组后极部巩膜MMP-2 mRNA表达显著增高，TIMP-2 mRNA表达降低，组间差异有显著性（P﹤0.01）。注射后随时间延长MMP-2 mRNA表达逐渐上调，48h达最高峰，至72h有降低。不同时间组组间差异显著（P﹤0.01），而TIMP-2 mRNA表达与之相反。自身对照组MMP-2 mRNA表达较同龄正常对照组有轻度上调，TIMP-2有下调，组间有统计学差异（P﹤0.01）。结论 外源性RA可以调节小鸡巩膜MMP-2/TIMP-2表达的平衡，并可能由此启动巩膜细胞外基质的主动重塑。
关键词 视黄酸，基质金属蛋白酶2 ，基质金属蛋白酶组织抑制剂，巩膜重塑
The Effect of Retinoic Acid in Changes of MMP-2 Expression and Avidity in the Posterior Sclera of Chick eye
Wang jianfeng, Liu shuangzhen, Wu wencan, Tan xingping, Jiang haibo Department of Ophthalmology, Xiangya Hospital in Central South University, Changsha 410008,
【Abstract】Objective To investigate the changes of matrix metalloproteinase 2(MMP-2) and its tissue inhibitor(TIMP-2) mRNA expression in posterior sclera of chick eye after a injection of RA. Methods 1-day-old white leghorn chicks were divided randomly and equivalently into three groups, RA was retrobulbar injected to the right eye of each chick to induce the experimental group, and the left eye served as self-control. NS injection induced the negative control group.Meanwhile, normal age-matched animals were provided as control group. Both eyes collected after chicks were killed at 12,24,48and72h after injection, and expression levels of MMP-2 and TIMP-2 mRNA were analyzed by single step reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with normal and self-controlled groups, the level of MMP-2 mRNA expression in the posterior sclera of the experimental group was high while that of TIMP-2 was low. There was statistical difference between experimental groups and control groups(P﹤0.01); As the time passed, levels of MMP-2 mRNA expression increased significantly and reached a peak at 48hour after injection, and then decreased slightly but still maintained in a relative high level. There was statistical differency between groups in different times(P﹤0.01).However, TIMP-2 mRNA expression was just contrary to that of MMP-2. There was statistical differency between normal and self-controlled groups (P<0.01), seemed as the RA injected effected by topediffusion. Conclusion Retinoic acid can effect on the expression of MMP-2 and TIMP-2 mRNA in the posterior segment of eye directly or indirectly. RA may be an important factor to switch on the extracellular matrix (ECM) remodeling of the posterior sclera.
Key words Retinoic Acid; Matrix metalloproteinase-2；Tissue inhibitor of matrix metalloproteinase-2；Extracellular matrix (ECM) remodeling, Sclera |
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