Purpose: Investigate changes in chondroitin sulfate proteoglycan (CSPG) distribution in Royal College of Surgeons (RCS) rat retinae. Could CSPGs distribution act as a physical barrier to transplanted cell migration in degenerating retinae?
Methods: CSPG expression was examined in RCS and Long-Evans rat retinae from birth to postnatal day 150 (PND150) using immunofluorescence and western-blots.
Results: CSPG-positive cells were localized in the ganglion cell layer (GCL) and the photoreceptor outer segment debris zone (DZ); CSPG expression in the DZ was the main contributor to the higher expression in older animals for both groups. Both groups showed a rapid rise in CSPG expression, for LE group, which peaked on PND21 before declining to lower levels by PND35, while for RCS group PND14 is the peak day before declining to lower lever. CSPG expression had risen again by PND90 and remained elevated for the duration of the study (PND150).CSPG expression was no significantly difference (P ≤ 0.05, n = 5) in rat retinae at the same age for both groups.
Conclusions: Increased expression of CSPGs in the DZ may act as a physical barrier following retinal cellular transplantation. CSPGs in the GCL is probably related to dendritic changes. CSPG accumulation in the older retinae suggests that aging in?uences the microenvironment in the retina, which may affect the efficacy of cell transplantation. |
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