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实验性视网膜脱离模型中RPE细胞粘附分子的表达 |
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| 实验性视网膜脱离模型中RPE细胞粘附分子的表达 |
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作者：陈慧瑾,马… 文章来源：北京大学第三医院,北京大学眼科中心 100083 点击数：866 更新时间：2006/6/19 19:19:36 
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| 目的：观察大鼠视网膜脱离及复位状态下视网膜色素上皮（RPE）细胞粘附分子的表达变化，探讨其与增殖性玻璃体视网膜病变（PVR）发生的关系。方法：通过视网膜下注射透明质酸钠的方法制造视网膜脱离的动物模型。在不同的时间点摘除眼球，制作冰冻切片，进行免疫组化及免疫荧光染色，比较RPE细胞上几种与细胞增殖和迁移密切相关的粘附分子，包括神经钙粘素、上皮钙粘素、整和素α5、整和素β1，以及纤维连接蛋白（FN）在正常视网膜、脱离的视网膜、复位的视网膜中表达的情况。进一步通过视网膜下注射神经钙粘素拮抗剂的方法确定视网膜脱离后神经钙粘素表达上调的意义。结果：视网膜脱离后，RPE细胞上的神经钙粘素、上皮钙粘素、整和素α5、整和素β1，以及纤维连接蛋白五种分子的表达都明显高于正常视网膜以及脱离后复位的视网膜。注射了神经钙粘素拮抗剂的视网膜，光感受器细胞外节缩短乃至消失，视网膜下细胞角蛋白阳性的细胞数目明显减少。结论：视网膜脱离会导致RPE细胞上几种重要的粘附分子的表达增加，视网膜复位可以逆转这种变化。视网膜脱离后神经钙粘素表达上调可能具有抑制光感受器细胞凋亡及促进RPE细胞迁移的作用。视网膜脱离可能是RPE细胞发生迁移以及PVR形成的始动因素，及时进行视网膜复位手术是预防PVR的根本方法.
ABSTRACT
PURPOSE To observe changes of adhesion molecules in retinal pigment epithelium (RPE) cells after experimental retinal detachment, in an attempt to explore the relationship between retinal detachment (RD) and proliferative vitreoretinopathy (PVR).
METHODS Rat retinas were detached by transcleral injection of sodium hyaluronate into the subretinal space. The eyes were enucleated at different intervals, followed by fixation, embedding, and sectioning. Immunohistochemistry and immunofluorescence was performed using antibodies against N-cadherin, E-cadherin, integrinα5, integrinβ1, and fibronectin. The difference of these adhesion molecules expression on RPE cells between the normal retina, detached retina, and reattached retina was observed. Furthermore, N-cadherin antagonist was injected into the subretinal space to determine the implication of upregulation of N-cadherin after RD.
RESULTS After RD, the expression of all five molecules was evidently increased on RPE cells. Whenever the retinas were reattached, the expression will decrease to the normal level. In the retinas in which N-cadherin was blocked, the photoreceptor outer segments were very short or even disappeared, and cytokeartin positive cells were markedly decreased.
CONCLUSIONS RD can result in increased expression of several adhesion molecules known to be associated with cell proliferation and migration, and this change can be reversed by retinal reattachment. The upregulation of N-cadherin after RD might contribute to the survival of photoreceptor and migration of RPE cells. RD could be the initiating factor for RPE cell migration and the onset of PVR. Surgical reattachment of the retina is the essential way to prevent PVR.
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| 会议投稿录入：chenhj 责任编辑：毛进 |
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上一篇会议投稿： 糖尿病视网膜病变共焦激光多普勒视网膜血流图的临床表现
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