|
|
 |
专题栏目 |
 |
|
相关文章 |
|
|
|
|
|
 |
Therapeutic effect on intraocular melanoma in mice with IL-12 gene modified tumor cell vaccine combinated with CpG-ODN |
热 |
|
| Therapeutic effect on intraocular melanoma in mice with IL-12 gene modified tumor cell vaccine combinated with CpG-ODN |
|
作者：Zhao jun… 文章来源： Shenzhen eye hospital（深圳市眼科医院）,518001 点击数： 更新时间：2006-6-30 15:15:47 
|
|
| Objective To observe the therapeutic effect on IL-12 gene modified tumor cell vaccine combinated with CpG oligodeoxynucletide（CpG-ODN）. Methods Model mice bearing tumor were divided into 3 groups, respectively phosphate-buffered saline (PBS) treatment group, IL-12 gene modified tumor cell vaccine (GTV) treatment group and GTV combinated with CpG-ODN (GTVC) treatment group. 30μl PBS, 30μl（3.0×106cells）inactivated GTV and GTVC containing 20μg CpG-ODN were respectively injected into subconjunctiva of mice bearing tumor 3 days and 10 days after bearing tumor mouse model had been established. The time of cornea diabrosis, metastases into cervical lymph nodes and lung, survival time and ratio of CD4+and CD8+T cells in peripheral blood were observed. Results The time of cornea diabrosis in GTVC and GTV groups was later than that in PBS group, but no statistical difference between GTVC and GTV group. Metastases in cervical lymph nodes of all mice were developed in PBS group, 25% mice in GTV group and in GTVC group 18 days after the model had been established. There was statistical difference in metastatic incidence respectively between PBS group and other groups. There was no statistical difference in metastatic incidence between GTV group and GTVC group. Metastases in lungs of all mice were developed in PBS group, 75% mice in GTV group and 13% mice in GTVC group 28 days after the model had been established. There was statistical difference in metastatic incidence respectively between GTVC group and other groups. Compared with negative control (NC) group, the ratio of CD4+and CD8+T cells in peripheral blood was lower in PBS and GTV groups. There was no statistical difference on the ratio between GTVC group and NC group. The survival time in GTVC group was longer than that in PBS and GTV groups, but no difference between PBS group and GTV group. Conclusions CpG-ODN can enhance the anti-tumor effect of IL-12 gene modified tumor cell vaccine. |
|
|
| 会议投稿录入：christinezeng 责任编辑：毛进 |
|
|
上一篇会议投稿： 表现为睫状体占位的Wegener肉芽肿一例
下一篇会议投稿： 慢性复发性葡萄膜炎51例诊治体会 |
|
|
| 【字体：小 大】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 |
|
 网友评论：（只显示最新10条。评论内容只代表网友观点，与本站立场无关！） |
| |
|
|
|
|
