A promising therapy for retinal diseases is to employ biodegradable scaffolds to deliver retinal progenitor cells (RPCs) for repairing damaged or diseased retinal tissue. In present study, cationic chitosan-graft-poly (ε-caprolactone)/polycaprolactone (CS-PCL/PCL) hybrid scaffolds were successfully prepared by electrospinning. Characterization of the obtained nanofibrous scaffolds indicated that zeta-potential, fibers diameter and the content of amino groups on their surface were closely correlated with the amount of CS-PCL inCS-PCL/PCL scaffolds. To assess the cell-scaffold interaction, mice retinal progenitor cells (mRPCs) were seeded on the electrospun scaffolds and cultured for 7 days. In vitro proliferation assays revealed that mRPCs grown on the CS-PCL/PCL (20/80) scaffolds were better than other electrospun scaffolds. Scanning electron microscopy (SEM) and real time quantitative PCR (qPCR) results showed that mRPCs grown on CS-PCL/PCL (20/80) scaffolds were more likely to differentiate towards retinal neurons as compared with mRPCs grown on PCL scaffolds. Taken together, our results suggest that potential utilization of CS-PCL/PCL (20/80) scaffolds for retinal tissue engineering.