|
|
 |
专题栏目 |
 |
|
相关文章 |
|
|
|
|
|
 |
Suppression of type I collagen expression by miR-29b via PI3K, Akt, and Sp1 pathway in Human Tenon’s Fibroblasts |
★★★ |
|
| Suppression of type I collagen expression by miR-29b via PI3K, Akt, and Sp1 pathway in Human Tenon’s Fibroblasts |
|
作者：李宁 文章来源：安徽医科大学第一附属医院 点击数：260 更新时间：2012/9/13 
|
|
Purpose To evaluate the expression profile of microRNAs (miRNAs) and their roles in human tenon’s fibroblasts (HTFs), and to establish a miRNA-based gene silencing method for antifibrosis in vitro.
Methods The miRNA expression profile was analyzed by microarray using quiescent and TGFbeta1-stimulated primary HTFs, respectively. Candidate miRNAs were identified by quantitative RT-PCR. miRNAs potentially targeting fibrosis-related genes were predicted using a published algorithm (TargetScan; Envisioneering Medical Technologies, St. Louis, MO). Predicted fibrosis-related genes regulated by candidate miRNAs were confirmed by transfection of the miRNA into HTF culture (with or without TGFbeta1 treatment), followed by quantitative RT-PCR and Western Blot.
Results Total of 38 miRNAs were identified to be upregulated, and 31 down-regulated, in TGFbeta1-stimulated HTFs. Among those, the miR-29b, down-regulated in TGFbeta1-treated HTFs, targeted a cadre of mRNAs that encode proteins involved in fibrosis, including PI3Kp85α, Sp1, and collagen type I alpha1 (Col1A1). Treatment of HTFs with TGFbeta1 activated the PI3K-Akt-Sp1 pathway and, consequently, induced an increase in the expression of type I collagen. Down-regulation of miR-29b by introducing an antisense miRNA into cultured HTFs partly induced the expression of PI3Kp85α, Akt, Sp1 and Col1A1, whereas overexpression of miR-29b inhibited the PI3K-Akt-Sp1 pathway and attenuated the expression of Col1A1.
Conclusions miR-29b acted as a suppressor of type I collagen gene by repressing the PI3K/Akt/Sp1 pathway in HTFs. Overexpression of miR-29b protected subconjunctival tissues against collagen production and fibrosis. These findings provided a novel rationale for the development of miRNA-based strategies for attenuating scar formation after glaucoma filtering surgery. |
|
|
| 会议投稿录入：毛进 责任编辑：毛进 |
|
|
上一篇会议投稿： 频域光学相干视网膜断层扫描对急性期Vogt-小柳-原田病的表现及转归的研究
下一篇会议投稿： 抗血管生成作用的VEGFxxxb家族在视网膜色素上皮细胞中的表达 |
|
|
| 【字体：小 大】【发表评论】【加入收藏】【告诉好友】【打印此文】【关闭窗口】 |
|
 网友评论：（只显示最新10条。评论内容只代表网友观点，与本站立场无关！） |
| |
|
|
|
|
