[Abstract] Objective: To study the expression of enhanced green fluorescence protein(EGFP) in cultured bovine trabecular meshwork cells(BTMC). Methods: Reanimating the pEGFP-C1 strain, pEGFP-C1 was extracted and identified. The pEGFP-C1 was embedded in lipofectamine and transfected into BTMC. The fluorescence intensity of BTMC after transfected with EGFP gene was visualized by fluorescence microscope. Results: pEGFP-C1 could be availably transfected into BTMC. After being transfected, BTMC with the EGFP gene displayed an effective EGFP expression. Conclusions: An EGFP expressing plasmid was used to transfected BTMC. Transfected cells show a stable EGFP expression in vitro. This labeling method will be profited for studying BTMC through genic level.
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